Nonstructural Protein 1 of Influenza A Virus Interacts with Human Guanylate-Binding Protein 1 to Antagonize Antiviral Activity
Figure 8
NS1 antagonized hGBP1-mediated antiviral activity.
A549 cells were transfected with the indicated plasmids. Transfectants were infected with PR8 virus at MOI = 1 after 24. Cells were collected at 24 hpi for analysis of viral replication. A. Viral titers in transfectants were measured by plaque assay. B. HA and NP mRNA in transfectants was analyzed by qRT-PCR. Results are means with SD from three independent experiments. *, p<0.05.