Impaired Neural Differentiation of Induced Pluripotent Stem Cells Generated from a Mouse Model of Sandhoff Disease
Figure 1
Characterization of iPSCs derived from the NSCs of Hexb–/− mice.
A, Phase-contrast image, AP staining and SSEA1 immunostaining (left: phase-contrast; right: immunostaining) of SD-iPSC clones (SFM1022, SFM6361, and SFM6362) grown on MEF feeder cells. EB3 (mouse ES cells) and WT-iPSCs were positive controls. Scale bar indicates 100 µm. B, RT-PCR analysis of ES cell marker gene expression in SD-iPSC clone SFM1022, WT-iPSCs, ES cells, and MEFs. Nat1 was used as an internal control. PCR products were amplified from cDNA samples with (+) or without (−) reverse transcriptase.