Thermally Targeted Delivery of a c-Myc Inhibitory Polypeptide Inhibits Tumor Progression and Extends Survival in a Rat Glioma Model
Figure 1
Plasma Clearance and Tumor Uptake of CPP-ELPs. A.
Plasma levels with time following IV injection of ELP1-Rho, SynB1-ELP1-Rho, or Bac-ELP1-Rho. Data represent the mean ± s.d. of 6 animals per group. B. Representative images of brain sections 4 h after IV injection of rhodamine-labeled polypeptides. C. Tumor levels 4 h after IV administration of rhodamine-labeled ELP or CPP-ELPs. Bars, s.e. *, Tumor levels are significantly enhanced (p<0.01, one way ANOVA with post hoc Bonferroni, n = 6 rats/group). D. Distribution of rhodamine-labeled polypeptides in tumor and normal brain relative to perfused vasculature. Rhodamine fluorescence was used to follow the localization of the polypeptide within the tumor (left panel), and the perfused vasculature was marked by infusion of high molecular weight dextran 1 min prior to euthanasia (middle panel). E. Microscopic images of tumor sections were collected after staining cell nuclei with Hoechst 33342 using a 60× magnification objective.