Peroxisome Proliferator Activated Receptor-α/Hypoxia Inducible Factor-1α Interplay Sustains Carbonic Anhydrase IX and Apoliprotein E Expression in Breast Cancer Stem Cells
Figure 7
ApoE is under the control the PPARα/HIF1α interplay in breast CSCs.
(A) ApoE mRNA qPCR analysis in N−/T-MS (samples 1–5) and in MCF10/MCF7-MS. (B) ApoE mRNA qPCR analysis in TAF supernantants (10%, 24 h), TNFα (0.75 ng/mL, 24 h)-exposed MCF7-MS. ApoE mRNA qPCR analysis in (C) pV/pPPARα (48 h)-transfected MCF7-MS and MCF10-MS, and in (D) HIF1 vector (48 h) or SCR/siHIF1 (72 h)-transfected MCF7-MS. Data are expressed as mean ±S.D., n = 3, *p<0.05, #p<0.01, §p<0.005, ANOVA test. n.s.: not significant.