Alterations in Gene Expression in Mutant Amyloid Precursor Protein Transgenic Mice Lacking Niemann-Pick Type C1 Protein
Figure 5
Transcript and protein expression levels of cathepsin B and cathepsin D in the hippocampus and cerebellum of five lines of mice.
A and C, Histograms showing increased mRNA levels for Ctsb (encoding cathepsin B, A) and Ctsd (encoding cathepsin D, C) in the hippocampus and cerebellum of Npc1-null and ANPC mice compared with WT control mice as obtained using customized real-time RT-PCR array. B and D, Immunoblotting performed to validate data obtained by PCR arrays revealed increased levels of cathepsin B (B) and cathepsin D (D) in the hippocampus and cerebellum of Npc1-null and ANPC mice compared with the WT mice. APP-Tg and Dhet mice showed no alteration in transcript or protein expression levels of cathepsin B and cathepsin D compared with WT mice. The protein levels of cathepsin B and cathepsin D were normalized to the β-actin and the values (n = 4 animals/genotype) are expressed as means ± SEM. *, p<0.05. CatB, cathepsin B; CatD, cathepsin D.