ATP-Mediated Transactivation of the Epidermal Growth Factor Receptor in Airway Epithelial Cells Involves DUOX1-Dependent Oxidation of Src and ADAM17
Figure 8
ATP stimulation induces DUOX1-dependent cysteine oxidation in Src.
(A) Serum-starved H292 cells were stimulated with ATP (100 µM) for indicated times and lysed in the presence of Iodoacetyl-LC-Biotin, followed by purification of biotin-labelled protein with NeutrAvidin agarose, and western blot analysis of biotinylated proteins and whole lysates for Src, reflecting Src-SH and total Src, respectively. (B) Densitometry analysis of Src-SH status from 3 separate experiments. *p<0.05 compared to untreated control. (C) Similar analysis of Src-SH status in H292-shDUOX1 or corresponding H292-CTL cells after 10-min stimulation with ATP (100 µM). (D) Densitometry analysis of Src-SH relative to total Src based on Western blot analysis in panel C. Mean values of 4 replicates from 2 independent experiments are shown. *p<0.05 compared to untreated control.