High-Resolution Analysis and Functional Mapping of Cleavage Sites and Substrate Proteins of Furin in the Human Proteome
Figure 2
Frequency plot of the cleavage sequence of furin in an IceLogo format.
The height of a character is proportional to the frequency of the amino acid residue at the individual position of the cleaved peptide and is normalized for the amino acid encoded in the entire human genome according to RefSeq. Because there was a five residue overlap between the P7-P1’ and P4-P4’ peptides from the individual cleavage site, the sequence logos represent the combined P7-P4’ peptide sequence. A. Peptide sequences with the Z-score over 3.5 for both P4-P4’ and P7-P1’ peptides (932 substrates). B. Peptide sequences with the Z-score for the P4-P4’ and P7-P1’ peptides over and below 3.5, respectively (64 substrates). C. Peptide sequences with the Z-score for the P4-P4’ and P7-P1’ peptides below and over 3.5, respectively (439 substrates). D. Peptide sequences for poor or no substrates with Z-score for the P4-P4’ and P7-P1’ peptides below 1.0 (391 sequences).