The V Protein of Tioman Virus Is Incapable of Blocking Type I Interferon Signaling in Human Cells
Figure 4
Expression of IFN-inducible genes is impaired by MuV-V but not TioV-V expression.
HEK-293T cells were transfected with pCI-neo-3xFLAG expression vectors encoding for 3xFLAG alone or fused to TioV-V, MuV-V, TioV-P or TioV-W (500 ng/well). 24 h after transfection, cells were left unstimulated or stimulated with 200 IU/ml of recombinant IFN-β. After 24 h of culture, total RNAs were extracted, and expression levels of indicated genes were quantified by qRT-PCR. For each gene, data were normalized so that 100% corresponds to cells transfected with pCI-neo-3xFLAG empty vector and stimulated with recombinant IFN-β (dotted red line). Experiment was performed twice and data represent means ± SD. *indicates that differences observed with MuV-V relative to controls cells transfected with 3xFLAG alone and stimulated with IFN-β were statistically significant (p-value<0.05).