The V Protein of Tioman Virus Is Incapable of Blocking Type I Interferon Signaling in Human Cells
Figure 2
Activation of ISRE-dependent gene expression by MeV or TioV infection.
HEK-293 cells stably transfected with an ISRE-luciferase reporter gene (STING-37 reporter cell line) were infected with MeV or TioV. (A) Bright field microscopy of cell cultures at 48 h post-infection (MOI = 1). (B) Luciferase expression was determined at 24 h and 48 h post-infection. (C) Culture supernatants from (B) were collected at 48 h post-infection, clarified by centrifugation, UV-inactivated and added to culture wells containing STING-37 cells. Alternatively, culture medium was supplemented with increasing doses of recombinant IFN-β. After 24 h, luciferase expression was determined. Luciferase activity in culture supernatants from (B) was below 1,400 luciferase activity units (data not shown). Experiment was performed in triplicates, and data represent means ± SD. *indicates that differences observed between MeV and TioV-infected cells were statistically significant (p-value <0.01).