Tim-3 Expression in Cervical Cancer Promotes Tumor Metastasis
Figure 3
Expressions of Tim-3 in cervical cancer cell lines.
(A) Tim-3 transcripts were detected in Hela and SiHa cell lines. The templates of different lanes as follows: Lane 1, total RNA of PBL; Lane 2, cDNA of PBL; lane 3, total RNA of SiHa; lane 4, cDNA of SiHa; lane 5,total RNA of Hela; lane 6, cDNA of Hela. (B) Tim-3 protein was determined by Western blotting in Hela and SiHa cell lines. (C) Hela cells stained with immunofluorescent with anti-Tim-3 antibody and observed with a confocal laser scanning microscope. Tim-3 protein (green, arrowheads) is in the cytoplasm of Hela. Cell nuclei (red) were visualized by staining with PI.