Monoclonal Antibodies against Hepatitis C Genotype 3a Virus Like Particle Inhibit Virus Entry in Cell Culture System
Figure 3
Effect of mAbs on HCV infection.
(A) JFH1 virus was preincubated with increasing concentrations (0.5, 5, 50, 100 µg/ml) of mAbs (E8G9 and H1H10) or 100 µg/ml of non-specific antibody F1G4 (Non sp) for 1 hr at 37°C before infecting Huh7.5 cells. Three days post infection, total cellular RNA was isolated and HCV negative strand level was measured using real time RT-PCR. GAPDH was used as an internal control. (B) mAb E8G9 was used in increasing concentrations to inhibit the virus entry and the input viral RNA present inside the cells (positive strand) was estimated 3 h post infection by real time RT-PCR. GAPDH was used as an internal control.