Depletion of Nuclear Poly(A) Binding Protein PABPN1 Produces a Compensatory Response by Cytoplasmic PABP4 and PABP5 in Cultured Human Cells
Figure 4
Level of different cellular PABPs in PABPN1 depleted cells.
HeLa cells were grown on 35 mm plates and transiently transfected with PABPN1 Si-RNA or PABPN1-UTR RNAi (A) Following transfection, cells were harvested in Laemmli buffer at 45 h, 66 h and 80 h. Whole cells extracts from NT (66 h), UTR (66 h), and PABPN1 Si (45 h, 66 h, and 80 h) transfected cells were analyzed for PABP1, 3, 4, 5 and PABPN1 by western blotting. GAPDH was used as loading control. (B) PABP5 abundance was calculated by scanning band intensities and expressed as an arbitrary unit. Data are mean ± SE, P<0.05, n = 3.