Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers
Figure 7
Secondary structure of recombinant (A) AgTx2 and (B) MgTx determined from NMR data.
Dipolar connectivities observed in 2D NOESY spectra are shown as double-sided red arrows. Cross-strand hydrogen bonds inferred from 1H-2H exchange rates are depicted as dashed orange lines. Green lines indicate disulfide-bonds inferred from NOE connectivities. The shaded arrows indicate the direction (N→C) of the three β strands.