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ESCRT-Independent Budding of HIV-1 Gag Virus-Like Particles from Saccharomyces cerevisiae Spheroplasts

Figure 3

HIV Gag-GFP VLP budding from yeast spheroplasts does not require Gag L-domains.

(A) and (B) Wild type yeast were transformed with , , , or . VLP budding of the Gag-GFP constructs was assayed as described in the Methods section. Gag-GFP was visualized by Western blotting of whole cell extracts and VLP fractions with anti-GFP antibody.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0052603.g003