Neutrophil-Derived Myeloperoxidase Aggravates Non-Alcoholic Steatohepatitis in Low-Density Lipoprotein Receptor-Deficient Mice
Figure 7
Attenuation of liver fibrosis in LDLR−/−/MPO−/−tp mice after 8 weeks high-fat diet.
A) Sirius red staining of liver sections of LDLR−/−/MPO−/−tp,LDLR−/−/MPO+/+tp, and chow-fed LDLR−/−/MPO+/+ mice (200× magnification). B) The amount of hydroxyproline, a protein modification specifically present in collagen and elastin, is significantly lower in the liver of LDLR−/−/MPO−/−tp mice in comparison with LDLR−/−/MPO+/+tp mice (3.3±0.2 vs. 4.6±0.7 nmol/mg tissue, p<0.01). C) LDLR−/−/MPO−/−tp mice display reduced expression of collagen type I, PAI-1, TIMP1, α-SMA, MMP-13, TGF-β1, and BAMBI, genes associated with hepatic fibrosis.