Fur Is a Repressor of Biofilm Formation in Yersinia pestis
Figure 1
Yersinia pestis biofilms assays.
a) Adherent bacterial biomass determined by crystal violet staining. Y. pestis was grown in the 24-well polystyrene dishes, and the biomass adherent to the well wall was stained with crystal violet to determine the OD570 values. The planktonic cells were subjective to determine the OD620 values (i.e., cell density) for normalization. Shown were the OD570/OD620 values representing the relative capacity of biofilm formation of each strain tested. b) Bacterial colony morphology. Aliquots of 5 µl of bacterial glycerol stocks were spotted on the LB plate, followed by the incubation for one week. c) Yersinia biofilms on C. elegans. The adult or L4 nematodes were spread on the lawn of Y. pestis expressing GFP and allowed to grow for 12 h. Shown were biofilms attach to the head posterior to the nematode mouth.