Loss of Atrx Sensitizes Cells to DNA Damaging Agents through p53-Mediated Death Pathways
Figure 1
Characterization of primary macrophage experimental cell system.
(A) Peritoneum-derived macrophage cell preparations isolated from Atrxf/f mice (left panel) were tested for their purity by staining with the monocyte/macrophage-specific marker CD14 (right panel). DIC - differential interference contrast image. Scale bar: 25 mM. (B) Macrophages infected with AdCre (black bars) or AdLacZ (white bars) were monitored for survival at 1 and 3 days post-infection. (C) Cells immunostained for Atrx 3 days following AdCre or AdLacZ infection. Cells were counterstained with DAPI. Scale bar: 50 mM. (D) Representative immunoblot for Atrx or β-actin 3 days following AdLacZ, AdCre or no treatment. (E) PCR amplification encompassing exon 18, the sequence targeted for deletion. Infection with AdCre but not AdLacZ results in efficient excision of exon 18.