A Novel Mouse Model for Multiple Myeloma (MOPC315.BM) That Allows Noninvasive Spatiotemporal Detection of Osteolytic Disease
Figure 6
Osteoclasts in bone marrow of NSG mice infiltrated with MOPC315.BM.Luc.
(A,B) Representative images of TRAP+ staining from the bone marrow of an age-matched control NSG mouse injected with RPMI 1640. (C–E) Representative images of TRAP+ staining from the bone marrow of an NSG animal injected with MOPC315.BM.Luc. Inset in (C) shows bone marrow localization of transplanted MOPC315.BM.Luc cells using staining for IgA. TRAP+ osteoclasts are indicated by black arrow heads. In all cases scale bar is 200 µm. (F) Quantification of osteoclasts per mm bone surface. Controls are age-matched RPMI-injected NSG mice.