Genomic Stability of Lyophilized Sheep Somatic Cells before and after Nuclear Transfer
Figure 5
γH2AX immunostaining in pronuclei of oocytes injected with up to four nuclei from freeze-dried lymphocytes.
NT = nuclear transfer, 4NT = 4 pronuclei (one fragmentated), PI = pronuclei stained with propidium iodide; γH2AX = phosphorylated H2AX, a marker of DSBs and, indirectly, of DNA repair; Merge = PI+γH2AX. Scale bar = 20 µm.