The Activation Pattern of Blood Leukocytes in Head and Neck Squamous Cell Carcinoma Is Correlated to Survival
Figure 2
Blood from HNSCC patients (n = 20) and controls (n = 20) was incubated with different Abs and analyzed with flow cytometry.
(A, B) Abs against CD3, CD69 and CD71 were used to distinguish activated total T cells. (C, D) To identify activated cytotoxic T lymphocytes (CTLs) CD69, CD71 and CD8 Abs were used. (E, F, G) CD4+ T helper (Th) cells were discriminated by CD4 Abs, and the activation status was established using CD69, CD71 and CD98 Abs. (H) Th2 cells were detected by CD4 and CRTH2 Abs, and the frequency of CD98+ Th2 cells was determined. (I) CD3, CD16, CD56 and CD69 Abs were used to discriminate CD69+ CD3−CD16+CD56+ natural killer (NK) cells. (J) Neutrophils were determined as CD16+ granulocytes, and the mean fluorescence intensity (MFI) of CD62L+ cells was calculated. (K) Staining of CD14, CD16 and CD62L was used to discriminate activation of the monocyte population CD14highCD16+. The MFI of CD62L+ CD14highCD16+ monocytes was established. * p≤0.05; ** p≤0.01; *** p≤0.001.