Genetic Loss of Murine Pyrin, the Familial Mediterranean Fever Protein, Increases Interleukin-1β Levels
Figure 2
Loss of Mefv does not affect the response to LPS.
A, Relative expression of Mefv in bone marrow-derived macrophages (BMMΦ), resting peritoneal macrophages (rpMΦ), and elicited peritoneal macrophages (pMΦ) from WT mice was detected by real-time PCR. n ≥3 mice per cell type. Data are expressed as relative expression normalized to Gapdh and BMMΦ. B-D, rpMΦ from Mefv+/+ and Mefv−/− littermate mice were untreated or treated with 1 µg/mL LPS for 24 h. B, Il1β and Il6 expression at the transcript level is expressed as fold induced by LPS treatment. n = 3 mice per genotype. C-E, Concentration of IL-6 and IL-1β in supernatants from LPS-treated rpMΦ cultures as determined by ELISA. n = 6 mice per genotype. E, The concentration of lactate dehydrogenase (LDH) released into cell supernatants was used as an indicator of cell death. WT and Mefv−/− mice were treated with 2.5 mg/kg (F, n = 8 WT and 5 Mefv−/− mice) or 0.25 mg/kg (G, n = 18 and 19) crude LPS by i.p. injection at time 0 h, as indicated by arrows. Rectal temperature at the indicated times is shown in F and G.