Different Characteristics and Nucleotide Binding Properties of Inosine Monophosphate Dehydrogenase (IMPDH) Isoforms
Figure 6
Nucleotides protect IMPDH in an isoform-specific manner via the Bateman domain.
(A) Representative Coomassie stained SDS-PAGE gel of a protease protection assay experiment, performed as outline in methods, with His-IMPDH2. INM stands for IMP, NAD and MPA. Molecular weight marker sizes are indicated. (B) Quantitation of remaining full-length protein from protease protection assay with His-IMPDH2 and His-IMPDH1 presented as percent protection. Shown is the mean ± SEM and the number of experiments (n) is indicated below the graph. (*p<0.05, **p<0.01, ***p<0.001 cf. the control (digested), # p<0.01 IMPDH1 cf. IMPDH2). (C) Representative gel of a protease protection assay experiment with His-Core2 protein. (D) Quantitated results as per B. Shown is the mean ± SEM from five independent experiments. (***p<0.001 cf. the control (digested), ## p<0.001 IMP, NAD and MPA cf. adenosine nucleotides).