TAK1 (MAP3K7) Signaling Regulates Hematopoietic Stem Cells through TNF-Dependent and -Independent Mechanisms
Figure 1
TAK1, TAB1 and TAB2 in bone marrow cells.
(A) Expression of TAK1, TAB1 and TAB2 proteins. Whole cell extracts of the BMN cells (BM), splenocytes (Spleen) and thymocytes (Thymus) were analyzed by SDS-PAGE and Western blotted (WB) using the indicated antibodies. The amount of β-actin is shown as a loading control. (B) Expression of Tak1, Tab1 and Tab2 mRNA. Total RNA was isolated from BMN cells (BM), splenocytes (Spleen) and thymocytes (Thymus), and analyzed by qPCR. Expression level of each gene was normalized to that of Actb and shown as the relative value to BM. Data are presented as mean ± S.D. of three independent experiments. (C) Expression of Tak1, Tab1 and Tab2 mRNA. The cells in the LT-HSC (CD34− Flt3− LSK), ST-HSC (CD34+ Flt3− LSK), MPP (CD34+ Flt3+ LSK), MP (Lineage− c-Kit+ Sca-1−) or Lineage+ fractions from wild type mouse bone marrow were sorted by FACS, and total RNA was prepared. The relative amounts of Tak1, Tab1, Tab2 and Actb mRNA were determined by qPCR. Expression level of each gene was normalized to that of Actb and shown as the relative value to LT-HSC. Data are presented as mean ± S.D. of four independent experiments.