Identification of Regions Involved in Substrate Binding and Dimer Stabilization within the Central Domains of Yeast Hsp40 Sis1
Figure 8
A) Model of the Sis1 binding and delivery.
A model that combines Sis1 observations showing that the disordered G/M region participates in the binding and delivery of client proteins to Hsp70. A schematic Sis1 structure is represented as a dimer showing its main domains and regions. Light blue, Sis1 CTD (PDB accession number 1C3G); red, Sis1 J-domain (PDB accession number 2O37); dark blue, Hsp70 NBD (PDB accession number 1HJO); green, DnaK SBD (PDB accession number 1DKX). The unstructured G/F and G/M regions of Sis1 are presented in black and red lines, respectively. The schematic substrate is in orange. B) Model of the Sis1 unfolding pathway. We propose a model for the Sis1 unfolding pathway which involves at least 2 events based on the results obtained in this study (see text for details).