PPAR-γ Activation Increases Insulin Secretion through the Up-regulation of the Free Fatty Acid Receptor GPR40 in Pancreatic β-Cells
Figure 5
PPAR-γ activation up-regulates β-cell gene expression through FoxO1 nuclear exclusion.
(A, B) Immunostaining (A) and immunoblot (B) of nuclear-cytoplasm shuttling for FoxO1 with RGZ treatment and/or PPAR-γ overexpression. (C) Immunoblot for β-cell specific gene expressions and FoxO1 shuttling with insulin receptor RNAi. (D) Immunoblot for β-cell specific gene expressions after treatment with adenovirus containing rat FoxO1 shRNA.