Compartmentalization of Mammalian Pantothenate Kinases
Figure 8
Mutagenesis of the NLS and NES of human PanK2(82–570).
(A) Schematic diagram of human PanK2 without the MTS and the mutant derivatives named: hPanK2-noNLS-mCherry and hPanK2-noNES-mCherry, each containing disrupted NLS or NES motifs, respectively. Arginine (R) or leucine (L) residues were replaced by the alanine (A) as indicated. (B) HeLa cells were transfected with expression plasmids encoding hPanK2(82–570)-mCherry (a, b), hPank2(82-570-noNLS)-mCherry (c, d), hPanK2(82-570-noNES)-mCherry (e, f) or mCherry alone (g,h). After 48 hours cells were stained with Hoetsch 33342 to visualize nuclei (green, b, d, f, h) and analyzed by live-cell confocal microscopy. Results are representative of at least 2 independent experiments. Scale bar, 10 µm.