Prion Seeding Activities of Mouse Scrapie Strains with Divergent PrPSc Protease Sensitivities and Amyloid Plaque Content Using RT-QuIC and eQuIC
Figure 7
PK-sensitivity of seeding activity in synaptosomes from 101LL mice infected with 263K and 139A.
5×10−6 (10−6) to 5×10−11 (10−11) dilutions of PK-treated (100 µg/mL) (C, D) or control (-PK) (A, B) detergent permeabilized synaptosomal fractions. 263K (A, C) and 139A (B, D) synaptosomal fractions were seeded into quadruplicate reactions. (E) End-point dilution RT-QuIC analysis of 263K (red bars) and 139A (orange bars) strains. The mean ±SD of Spearman-Kärber estimates of the SD50/mg brain tissue from three different experiments are shown.