HIF-1α Inhibition Reduces Nasal Inflammation in a Murine Allergic Rhinitis Model
Figure 5
Immunohistochemical and Western blot analysis of HIF-1α and VEGF expression in OVA-challenged mice.
(A and B) Immunostaining for HIF-1α and VEGF in nasal mucosa following the last challenge. Positive HIF-1α signals are brown and are predominantly nuclear and VEGF is predominantly cytoplasmic (magnification 400×, scale bar = 20 µm). (C and D) Representative Western blot analysis showing HIF-1α and VEGF (with β-actin as a loading control) expression in the nasal mucosa 24 h after the last challenge. All densitometric analyses are presented as the relative ratio of each molecule to β-actin and the ratio in negative control mice was set to 100. Values represent means±SEM (n = 6 mice). *Significantly different from negative control, p<0.05; #significantly different from positive control, p<0.05.