Prostatic Acid Phosphatase Is Required for the Antinociceptive Effects of Thiamine and Benfotiamine
Figure 7
Pharmacokinetic profiles of thiamine, TMP and TDP do not differ between wild-type and Pap−/− mice following oral administration of BT.
(A–F) BT (0–300 mg/kg, p.o.) was administered to wild-type and Pap−/− mice (n = 5/dose). The indicated tissues were harvested 1 h post oral gavage then were homogenized in 10% trichloroacetic acid. Fluorescent thiochrome derivatives were monitored by reversed phase HPLC to detect (A–B) thiamine, (C–D) TMP, and (E–F) TDP. Peak area values were converted to units of concentration for each thiamine derivative and normalized by the weight of tissue in each replicate. There were no significant differences between wild-type and Pap−/− mice. Data are plotted as means ± s.e.m.