Establishment and Characterization of a Highly Tumourigenic and Cancer Stem Cell Enriched Pancreatic Cancer Cell Line as a Well Defined Model System
Figure 4
Clonogenic potential and tumourgenicity.
Clonogenic potential and tumourgenicity of JoPaca-1 were determined by limiting dilution assays in vitro and in vivo. A. Dilution of cells in a 96-well plate and counting of colony-containing cells after 10 days lead to a clonogenic potential of 1 cell in 48 as calculated by ELDA [32]. B. Orthotopic injection of 10.000, 1.000, and 100 JoPaca-1 cells into 6 NOD.Cg-Prkdcscid Il2rgtm1Wjl mice each resulted in formation of tumours which were excised, weighted and photographed. Average tumour masses are shown.