Heme Binding Proteins of Bartonella henselae Are Required when Undergoing Oxidative Stress During Cell and Flea Invasion
Figure 1
Strains XL1-Blue pBAD24 and XL1-Blue pBAD24 expressing Hbps were expressed for 2 h at 37°C as described in Materials and methods. Expression was evident in SDS-PAGE (A). Line 1: XL1-Blue pBAD24, lines 2 to 5: XL1-Blue pBAD24 expressing HbpA, B, C, D, respectively (20 µg). After plating on Congo red plates supplemented with 0.2% arabinose, strains were grown for 20 h at 37°C. Strain XL1-Blue pBAD24 (B) formed white colonies. Strain XL1-Blue pBAD24::hbpA (C), XL1-Blue pBAD24::hbpB (D), XL1-Blue pBAD24::hbpC (E) and XL1-Blue pBAD24::hbpD (F) formed red colonies.