DNA Hypomethylation Affects Cancer-Related Biological Functions and Genes Relevant in Neuroblastoma Pathogenesis
Figure 1
DNA methylation profiling and identification of differentially methylated genes in NB.
A: Unsupervised Principal Component Analysis (PCA) of array-based DNA methylation data in 21 neuroblastomas (NB) (classified according to INSS Stage), 2 ganglioneuroma (GN), 1 ganglioneuroblastoma (GNB); and normal reference samples: fetal brain (FB) and adrenal gland (AG). B: Venn diagram showing the strategy used to identify NB-Specific genes. Hypermethylated and hypomethylated genes in NB were determined using three different supervised analyses with distinct reference samples (FB, AG and GN/GNB). C: Supervised hierarchical cluster analysis of DNA methylation data from NB-specific genes in 21 NB samples, 2 GN, 1 GNB; and 2 normal reference samples: FB and AG. D: Supervised Principal Component Analysis (PCA) in 21 NB samples, 2 GN, 1 GNB; and normal reference samples: FB and AG for NB-specific genes.