Comparison of the Kinetics of Maturation of Phagosomes Containing Apoptotic Cells and IgG-Opsonized Particles
Figure 2
Effect of PS enrichment in binding and phagocytosis of agRBC by SMC.
After aging and their incubation with different ratios of RBC:PS the cells were labeled and added to the SMC. A) Effect of PS enrichment in binding of the agRBC to SMC as a function of time. SMC were allowed to bind RBC on ice, and then warmed up for 35 s as described in Experimental Procedures. The cells were then fixed and analyzed by light microscopy. The values show the number of RBCs bound per 100 phagocytic cells. B) Effect of PS enrichment in phagocytosis of agRBC. The values show the percentage of SMC that have internalized phagocytic particles (Phagocytic Index). C) Effect of PS enrichment in the number of RBC ingested per phagocytic cell. Results are expressed as mean ± SD of at least three independent experiments. At each time point 100 phagosomes were analyzed. D-G) Representative images of agRBC phagocytosis by a SMC at 0 min chase time. D) Actin staining with Rhodamine-Phalloidin (in red). E) Engulfed agRBCs stained with CFSE (in green). F) Nucleus staining with DAPI (in blue). G) Corresponding merged image. Bar, 10 µm.