Neuroprotective Actions of Methylene Blue and Its Derivatives
Figure 2
Neuroprotective effect of MB against glutamate neurotoxicity in HT-22 cells.
(A) MTT viability assay (B) and Calcein AM viability assay at 12 hours after 20 mM glutamate insult demonstrate the dose dependent neuroprotective effect of MB. * p<0.05 compared to 20 mM glutamate media (C) Representative Calcein AM fluorescent images depict the neuroprotective effect of MB (1 µM) in HT-22 cells after 12 hours exposure of 20 mM glutamate (scale bar 200 µm). (D) Total GSH assay demonstrates that MB has no effect on the glutamate-induced GSH depletion. * p<0.05 compared to vehicle control.