E2F6 Associates with BRG1 in Transcriptional Regulation
Figure 4
Chromatin immunoprecipitaion of E2F6 and BRG1 on G1/S and G2/M promoters.
E2F6 and BRG1 bind to G1/S promoters but not G2/M promoters. Cells were synchronized by serum deprivation and then collected at 24 hours following serum stimulation. Chromatin immunoprecipitation was performed with antibodies to E2F6 and BRG1. A rabbit IgG was used as a negative control. Enrichment for G1/S and G2M promoters were assessed by quantitative PCR using SYBR Green.