E2F6 Associates with BRG1 in Transcriptional Regulation
Figure 2
BRG1 interacts with the E2F4 and E2F6.
BRG1 specifically interacts with E2F4 and E2F6 but not with the activator E2Fs. A plasmid construct expressing BRG1 was cotransfected into 293 cells with plasmid constructs expressing HA-tagged E2F1, E2F2, E2F3, E2F4 or E2F6. Immunoprecipitation experiments were carried out in lysis buffer with a polyclonal antibody recognizing the HA epitope tag. Immunoprecipitates were resolved on SDS PAGE and Western blotted with a BRG1 monoclonal antibody.