SIV Genome-Wide Pyrosequencing Provides a Comprehensive and Unbiased View of Variation within and outside CD8 T Lymphocyte Epitopes
Figure 3
Variation in CD8-TL epitopes in a virus population from an M3/M3 elite controller.
Amino acid variation detected by pyrosequencing in 12 CD8-TL epitopes in viruses isolated from CY0165 is compared to variation previously reported by Sanger sequencing bulk PCR amplicons [18]. Sequences that match the inoculum are represented with a “.”. The number of high quality reads with each specific sequence is shown. The total number of reads for each epitope can be found in Table S2. The frequency of reads with a specific amino acid sequence is shown as “% Var.” The sequences labeled as “other” include variants that were individually present at less than 1% and variants that were also detected in the inoculum. The relative shading of each box reflects the frequency of reads, as indicated in the legend.