Rotavirus Infection of Cells in Culture Induces Activation of RhoA and Changes in the Actin and Tubulin Cytoskeleton
Figure 6
Effect of calcium chelation or silencing the expression of NSP4 on changes in the actin cytoskeleton of MA104 cells infected with rotavirus.
Cells were either mock infected (A) or infected (B–E) with the reassortant rotavirus strain DxRRV at a m.o.i. of 10; at 7 h.p.i. cells were fixed and processed for immunofluorescence. Infected cells were either left untreated (B), treated with the indicated final BAPTA concentration 2 h before fixation (C), or transfected with siRNANSP4 before infection (D and E). Infected cells transfected with siRNANSP4 were stained either with antibody to NSP4 (D) or to VP6 as primary antibody (E). Note the presence of stress fibers, but not disorganized actin, in cell either treated with BAPTA or transfected with siRNANSP4. Scale bar, 5 µm.