Potent and Specific Antitumor Effect for Colorectal Cancer by CEA and Rb Double Regulated Oncolytic Adenovirus Harboring ST13 Gene
Figure 2
Colorectal cancer specific antitumor effect of Ad·(ST13)·CEA·E1A(Δ24) in vitro analyzed by the MTT assay. A.
The viability of tumor cells infected with different MOIs of the various oncolytic adenoviruses. Three CRC tumor cell lines (SW620, HCT116 and HT29), and three CEA-negative cell lines (Bcap37 breast cancer, CNE Nasopharynageal carcinoma and HeLa cervical carcinoma) and two normal cells (QSG7701 and WI38) were infected with either Ad·(ST13)·CEA·E1A(Δ24), Ad·(EGFP)·CEA·E1A(Δ24), or the typical oncolytic virus ONYX-015 at a range of MOIs (0.1, 1, 5 or 10 MOI), 4 days, cell viability was determined using an MTT assay. Uninfected cells were considered to be 100% viable. Bars represent the means ± SD (n = 6). B. The influence of viral infection on cell viability at different times. Three CEA positive cell lines (SW620, HCT116, and HT29) and three CEA-negative cell lines (Bcap37, CNE and HeLa) and two normal cells (QSG7701 and WI38) were infected with either ONYX-015, Ad·(EGFP)·CEA·E1A(Δ24), or Ad·(ST13)·CEA·E1A(Δ24) at an MOI of 10. After 24, 48, 72, and 96 hours, the cell viability was measured using the MTT assay. The data are presented as the mean ± SD of triplicate experiments. C. The viability of tumor cells infected with different MOIs of Ad·(ST13)·CEA·E1A(Δ24). CEA-negative colon cancer cell line (Colo-320) and CEA-positive non-colon cancer cell line (A549, MCF-7) were infected with Ad·(ST13)·CEA·E1A(Δ24) at a range of MOIs (0.1, 1, 5 or 10 MOI), 3 days, cell viability was determined using an MTT assay. Bars represent the means ± SD (n = 6).