In Vitro and Ex Vivo Inhibition of Human Telomerase by Anti-HIV Nucleoside Reverse Transcriptase Inhibitors (NRTIs) but Not by Non-NRTIs
Figure 8
Continuous treatment of HT29 cells with the guanosine analog ABC causes telomere shortening. A.
TRF blots of untreated (left) and ABC-treated (right) HT29 cells. PDL at which TRF was analyzed is shown above each lane. Molecular mass markers are shown at left and right of gel images. Each TRF smear was quantified as a weighted average and is shown below each lane. B. Growth curves of HT29 cells treated continuously with ABC. The growth curve of untreated HT29 cells (blue line) is plotted for comparison. C. Telomere length maintenance dynamics. The TRF dynamics of untreated HT29 cells (blue line) is plotted for comparison. Loss of telomere length is observed with the two lower doses (12.5 µM and 50 µM) of ABC treatments, with an apparent dose-response relationship. Cellular toxicity induced by the treatment with the highest (100 µM) ABC dose prevents longer-term TRF analysis, but substantial TRF loss was observed at PDL2.