Inflammatory Cytokines and Survival Factors from Serum Modulate Tweak-Induced Apoptosis in PC-3 Prostate Cancer Cells
Figure 2
Fn14 expression in human prostate cancer PC-3 and LNCaP cell lines.
A) Analysis of Fn14 and TWEAK mRNA expression by quantitative RT-PCR. Mean (±SEM) of three independent experiments; *p<0.05 vs LNCaP cells. B) Analysis of Fn14 protein expression by western blot in human prostate cancer cells cultured with or without 10% FBS for 24 hours. Mean (±SEM) of three independent experiments.*p<0.02 vs LnCAP cells. †p<0.05 vs 0% FBS PC-3 cells. HK2 cells are shown as positive control. C) Analysis of cell surface expression of Fn14 by flow cytometry. Representative experiment. Cells were stained with an isotype-matched antibody (white area) or anti-Fn14 antibody (black area).