Activation of AMP-Activated Protein Kinase by 3,3′-Diindolylmethane (DIM) Is Associated with Human Prostate Cancer Cell Death In Vitro and In Vivo
Figure 2
AMPK inhibitor Compound C can block AMPK activation by B-DIM in prostate cancer cells.
Prostate cancer C4-2B and LNCaP cells were pre-treated with 20 µM of AMPK inhibitor Compound C (CC) for 6 hours, followed by co-treatment with indicated concentrations of B-DIM for 3 hours. Cell extracts of the treated cells were immunoblotted for anti-phosphor-AMPKα, phosphor-ACC or β-actin antibodies.