Divergent Resorbability and Effects on Osteoclast Formation of Commonly Used Bone Substitutes in a Human In Vitro-Assay
Figure 4
Resorption of biomaterial and dentin and determination of the RRC.
A) Representative scanning electron microscopy (SEM) images of assessable biomaterials at day 0 and 28 (left panel) and toluidine blue stained dentin chips on day 28 (right panel) of osteoclast differentiation. Scale bars 50 µm. Note the smooth and regular surface of biomaterials at day 0, allowing precise determination of biomaterial resorption after 28 days of osteoclast culture. B) Quantification of biomaterial (BM, upper panel) and dentin (middle panel) resorption (mean resorption per osteoclast) and calculation of the relative resorption coefficient (RRC). Control is indicated as a dotted red line. Bars represent mean ± SD of four independent experiments with PBMCs from two different donors. Superscripts indicate statistically significant differences (*p<0.05 vs. control; #p<0.05 vs. Biobon).