The Mitochondrial Na+/Ca2+ Exchanger Upregulates Glucose Dependent Ca2+ Signalling Linked to Insulin Secretion
Figure 6
Effect of NCLX silencing expression on ATP production and insulin secretion.
A. Effect of NCLX silencing expression on ATP production. The ATP content was determined in pancreatic primary β cells lysates transfected with either siNCLX or siControl and stimulated with high glucose in the indicated times (see Experimental Procedures), n = 3 (*P<0.05). B. Effect of NCLX knocked down expression on glucose dependent insulin secretion. Cultured pancreatic primary β cells were transfected with either siNCLX or siControl and amounts of secreted insulin were determined in the indicated times, n = 3 (*P<0.05).