The Epithelial Sodium Channel (ENaC) Establishes a Trafficking Vesicle Pool Responsible for Its Regulation
Figure 5
. (A) Representative ISC trace from FTR epithelial cells transfected with α,β,γENaC (black trace) or control plasmid (grey trace), mounted in Ussing chambers and stimulated with 10 µM forskolin (grey bar). The expression of ENaC was confirmed by the addition of 10 µM amiloride (black bar) at the end of the recording. (B) Data from a number of similar experiments (n = 34) are summarized. Basal (-cAMP) and forskolin-stimulated (+cAMP) INa are presented on the left and the change in ISC with foskolin stimulation (ΔISC) on the right (bar graph). (C) Representative capacitance trace from the same samples provided in (A). (D) Summarized data for capacitance changes under basal and forskolin-stimulated conditions as in (B). (* - indicates significantly different from untransfected p<0.05).