Erioflorin Stabilizes the Tumor Suppressor Pdcd4 by Inhibiting Its Interaction with the E3-ligase β-TrCP1
Figure 1
Erioflorin stabilizes Pdcd4(39–91)luc from TPA-induced degradation.
(A) Structure of erioflorin (Mwt = 348.4). (B) Stably Pdcd4(39–91)luc expressing HEK293 cells were treated for 8 h with TPA (10 nM) with increasing concentrations of erioflorin (0.0625–10 µM). Pdcd4 stabilizing activity was determined relative to Δ(RLUcontrol–RLUTPA-only). (C) Stably Pdcd4(mut39–91)luc expressing HEK293 cells were treated as in (B). Luciferase activity is given relative to TPA-treated controls. All data are presented as means ± SEM (n≥3, *p<0.05, **p<0.01).