Metabolic Consequences of High-Fat Diet Are Attenuated by Suppression of HIF-1α
Figure 8
Accumulation of glycogen during HIF-1α ASO treatment.
(A) Representative images of liver sections in HIF-1α ASO treated (upper panel), control ASO treated (middle panel) and untreated mice (bottom panel). Periodic acid Schiff (PAS) staining, x 400 original magnification. HIF-1α ASO treated mice showed intense PAS positive staining, which was absent in other groups. Control ASO treated and untreated mice show macrovesicular steatosis, which was attenuated in HIF-1α ASO treated mice. (B) Glycogen levels in livers measured biochemically (see Methods). * and † denote p<0.05 and <0.01, respectively, for the difference with HIF-1α ASO treated mice. (C) Western blots showing phosphorylation of glycogen synthase kinase (GSK) α in HIF-1α ASO treated, control ASO treated and untreated mice in representative samples. GAPDH, glyceraldehydes-3-phosphate dehydrogenase.