Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Figure 3
EAAT4 (A, C, E) and EAAT5 (B, D, F) protein localization in mouse tissue.
Western blots using antibodies against EAAT4 (A, ∼61 kD) or EAAT5 (B, ∼62kD). EAAT4 is expressed in the cerebellum (Cbl), vestibular ganglia (VG) and vestibular organs (VE). EAAT5 is expressed in the retina (Rt), vestibular ganglia (VG) and vestibular organs (VE). Immunohistochemistry on utricular sections shows that EAAT4 (C) and EAAT5 (D) labeling (red, top and bottom panels) is conspicuous below the nuclei of both types of hair cells (I, II), where ribbon synapses are abundant. Calretinin labeling (green, top panels) marks type II hair cells (II) [33], [45], [46]. Immunohistochemistry of vestibular ganglion cells shows weak EAAT4 (E) and EAAT5 (F) labeling. Scale bars: 10 µm.