Differential Effects of IL-12 on Tregs and Non-Treg T Cells: Roles of IFN-γ, IL-2 and IL-2R
Figure 10
IL-27 inhibits IL-2 production, but only modestly affects IL-2R expression by Tconv and CD8 T cells.
Lymphocytes were prepared from naïve B6 mice and treated with anti-CD3 mAb and IL-27 (100 ng/ml) for 66 hr. (A) The fraction of CD4 and CD8 T cells expressing IL-2 was diminished. Data are from 3 independent experiments. (B) IL-27 diminished Foxp3 GMFI in Tregs. Samples were analyzed in triplicate. Data are from one experiment representative of 3 independent experiments. (C, D, E) Lymphocytes were labeled with CFSE prior to incubation. IL-27 reduced proliferation of Tregs and Tconvs but not CD8 T cells (C, D), resulting in a modest decrease in the percentage of Foxp3+ Tregs (E). Data are from 3 independent experiments. (F) IL-27 treatment resulted in small changes in CD25 expression on Tregs and CD8 T cells but not Tconvs and in a small decrease in CD122 expression on all three cell types. Data are representative of 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001, Student’s two-tailed un-paired t tests (A, B, E) or paired t tests (D).