Differential Effects of IL-12 on Tregs and Non-Treg T Cells: Roles of IFN-γ, IL-2 and IL-2R
Figure 8
IL-12 inhibits IL-2 expression by T cells.
Lymphocytes were prepared from naïve Foxp3gfp mice and treated with anti-CD3 mAb and IL-12 or IFN-γ. (A). The fraction of CD4 and CD8 T cells expressing IL-2 was diminished after 48 and 66 hr. Data are from 3–5 independent experiments. (B) Levels of IL-2, measured by ELISA, were reduced in IL-12 but not IFN-γ-treated cultures at 48 hr. Data are representative of 2 independent experiments performed in triplicate for stimulation, and each sample plated in duplicate for ELISA. *P<0.05, ***P<0.001, Student’s two-tailed un-paired t tests.